Immortalized Human Fibroblast Cell Line

Human foreskin fibroblasts hff were immortalized via retrovirus mediated gene transfer of the e6 and e7 genes of human papillomavirus type 16.

Immortalized human fibroblast cell line.

Immortalised cell lines are widely used as a simple model for more complex biological systems for example for the analysis of the biochemistry and cell biology of mammalian including human cells. The cells can be grown indefinitely in culture. Immortalized cell lines are either tumorous cells that do not stop dividing or cells that have been artificially manipulated to proliferate indefinitely and can thus be cultured over several generations table 14 1 because immortalized cells continuously divide they eventually fill up the dish or flask in which they grow. Htert immortalized primary cells atcc human telomerase reverse transcriptase htert immortalized cell lines represent a breakthrough in cell biology research that combines the in vivo nature of primary cells with the traditional cell line s ability to survive continuously in vitro.

Typical fibroblastoid morphology. Response to tgfß treatment by induction of α sma expression. Htert immortalized human foreskin fibroblast cell line. The primary mouse embryonic fibroblast cells were transferred the t every 3 days the first 3 and inoculated at the rigid density of 3 10 5 cells per 20 cm 2 dish the second 3 continuously.

By passaging also known as splitting scientists transfer a. The spontaneously immortalized cells with stable growth rate were established after 20 to 30 generations in culture and then named 3t3 cells. Expression of typical mesenchymal marker vimentin. Atcc was entrusted with its first cell line in 1962 and has consistently attained the highest standards and used the most reliable procedures to verify every cell line since.

The if cell line was evaluated for permissiveness to human cytomegalovirus hcmv infection after the if cell line surpassed the normal. The main advantage of using an immortal cell line for research is its immortality.